In this step a coarse-grain evaluation of the scaffold protein is performed in order to understand what are the most relevant elements for defining the dynamics behavior of the protein. The flexibility evaluation has three different options to choose from. Depending on the situation, the user might require one or multiple methods for computationally assessing in the flexibility: Anisotropic Network Model (ANM), and Gaussian Network Model (GNM) are automatically calculated by the server. At the same time, the user may use the information already present in the PDB file (as the B-factor value) and/or upload a file with their own calculated b- factors, for instance from Molecular Dynamics simulations. If more than one flexibility evaluation is used, the correlation in between different methods is shown after the evaluations are done.