From 2a2fb34aed013e73b2c0564884f8b15862c37fdc Mon Sep 17 00:00:00 2001
From: Giovanni Bussi <giovanni.bussi@gmail.com>
Date: Tue, 6 Jun 2017 08:00:54 +0200
Subject: [PATCH] Improved trieste-5

Added solutions and figures.

Fixes #237
---
 user-doc/figs/trieste-5-colvars.png           | Bin 0 -> 24168 bytes
 user-doc/figs/trieste-5-demux-bad.png         | Bin 0 -> 14278 bytes
 user-doc/figs/trieste-5-demux-good.png        | Bin 0 -> 32867 bytes
 user-doc/tutorials/a-trieste-5.txt            |  47 +++++++++++++++---
 .../tutorials/trieste-5/.solutions/driver.dat |   9 ++++
 .../trieste-5/.solutions/plumed2_reweight.dat |   2 +-
 .../trieste-5/.solutions/plumed3.dat          |  24 +++++++++
 .../trieste-5/.solutions/plumed3_reweight.dat |  35 +++++++++++++
 8 files changed, 110 insertions(+), 7 deletions(-)
 create mode 100644 user-doc/figs/trieste-5-colvars.png
 create mode 100644 user-doc/figs/trieste-5-demux-bad.png
 create mode 100644 user-doc/figs/trieste-5-demux-good.png
 create mode 100644 user-doc/tutorials/trieste-5/.solutions/driver.dat
 create mode 100644 user-doc/tutorials/trieste-5/.solutions/plumed3.dat
 create mode 100644 user-doc/tutorials/trieste-5/.solutions/plumed3_reweight.dat

diff --git a/user-doc/figs/trieste-5-colvars.png b/user-doc/figs/trieste-5-colvars.png
new file mode 100644
index 0000000000000000000000000000000000000000..46efc381a2b2924844fb3734702fffd38fb3eb20
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diff --git a/user-doc/tutorials/a-trieste-5.txt b/user-doc/tutorials/a-trieste-5.txt
index 082c79e5e..be0a2eff0 100644
--- a/user-doc/tutorials/a-trieste-5.txt
+++ b/user-doc/tutorials/a-trieste-5.txt
@@ -29,6 +29,9 @@ advantage of a special syntax for setting up multi-replica simulations that is o
 since version 2.4. Exercizes could be done also with version 2.3 but using a different syntax
 with respect to the one suggested.
 
+Also notice that in the `.solutions` directory of the tarball you will find correct input files.
+Please only look at these files after you have tried to solve the problems yourself.
+
 \section trieste-5-introduction Introduction
 
 So far we always used PLUMED to run one simulation at a time. However, PLUMED can also
@@ -414,9 +417,9 @@ two free-energy wells that correspond roughly to positive and negative \f$\phi\f
 This will tell you the unbiased population of the two minima. You should expect
 values close to these ones
 \verbatim
-0.978318 0.0216822
+0.968885 0.0311148
 \endverbatim
-that tells you that the first minimum has a population of roughly 98%.
+that tells you that the first minimum has a population of roughly 97%.
 
 \note
 This approach can be used to reweight any replica exchange simulation, irrespectively of how
@@ -440,9 +443,33 @@ but using only three replicas:
 - two unbiased replicas.
 - one biased replica along psi.
 
-Compute as before the relative population of the two minima. Which is the result? What can you learn from this example?
+Create a file `plumed3.dat` aimed at this. If you are not able you can find a working one in the `solutions` directory.
+Then use the following commands similarly to before:
+\verbatim
+> mpirun -np 3 gmx_mpi mdrun -plumed plumed3.dat -nsteps 1000000 -replex 120 -multi 3
+> gmx_mpi trjcat -f traj_comp{0,1,2}.xtc -cat -o fulltraj.xtc
+> mpirun -np 3 plumed driver --mf_xtc fulltraj.xtc --plumed plumed3_reweight.dat --multi 3
+> paste COLVAR_WHAM.{0,1,2} | grep -v \# | awk '{for(i=1;i<=NF/2;i++) printf($(i*2)"  ");printf("\n");}' > ALLBIAS
+> python3 SCRIPTS/wham.py ALLBIAS 3 2.5
+\endverbatim
+
+Compute as before the relative population of the two minima.
+\verbatim
+> paste <(grep -v \# COLVAR_CONCAT.0) weights.dat | awk '{if($2>0)wb+=$NF; else wa+=$NF}END{print wa,wb}'
+\endverbatim
+Which is the result? What can you learn from this example?
+
+If you did things correclty, here you should find a population close to 2/3 for the first minimum and 1/3 for the second one.
+Notice that this population just reflects the way we initialized the simulations (2 of them in one minimum and 1 in the other minimum)
+and does not take into account which is the relative stability of the two minima according to the real potential energy function.
+In other words, we are just obtaining the relative population that we used to initialize the simulation.
+
+Also plot the colvar files and look at them with attention. An excerpt is shown below.
 
-Also plot the colvar files and look at them with attention. How many transitions between the two free-energy wells can you observe?
+\image html trieste-5-colvars.png "Time series of phi from the simulations with a missing variable (exercise 3). Notice that there are appaernt transitions, but they are
+always due to accepted exchanges between replicas."
+
+How many transitions between the two free-energy wells can you observe?
 Remember that replica exchange involves coordinate swaps that do not correspond to the real system dynamics. It is very useful to look at
 "demuxed" trajectories.
 
@@ -464,9 +491,17 @@ continuous ones with the following commands
 
 You will now find new trajectories `0_trajout.xtc`, `1_trajout.xtc`, `2_trajout.xtc`, and `3_trajout.xtc`.
 These files can be in turn analyzed with `plumed driver`. Try to recompute collective variables on the trajectories
-obtained in \ref trieste-5-ex-1 and with those obtained in \ref trieste-5-ex-3. Can you spot out the difference between
-the two cases?
+obtained in \ref trieste-5-ex-1 and with those obtained in \ref trieste-5-ex-3. 
+
+\image html trieste-5-demux-good.png "Time series of phi obtained from the demuxed trajectories from exercise 2"
+\image html trieste-5-demux-bad.png "Time series of phi obtained from the demuxed trajectories from exercise 3"
+
+As you can see, the trajectories from \ref trieste-5-ex-1 show a number of transitions. On the other hand, the trajectories from \ref trieste-5-ex-3
+are stuck.
 
+As a general rule, notice that there is no way to estimate correctly the relative population
+of two metastable states if there is not a continuous "demuxed" trajectory joining them,
+possibly exhibiting multiple transitions.
 
 \section trieste-5-conclusion Conclusions
 
diff --git a/user-doc/tutorials/trieste-5/.solutions/driver.dat b/user-doc/tutorials/trieste-5/.solutions/driver.dat
new file mode 100644
index 000000000..565bb1985
--- /dev/null
+++ b/user-doc/tutorials/trieste-5/.solutions/driver.dat
@@ -0,0 +1,9 @@
+# load the pdb to better select torsions later
+MOLINFO STRUCTURE=reference.pdb
+# Compute the backbone dihedral angle phi, defined by atoms C-N-CA-C
+phi: TORSION ATOMS=@phi-2
+# Compute the backbone dihedral angle psi, defined by atoms N-CA-C-N
+psi: TORSION ATOMS=@psi-2
+
+# Just compute the collective variables 
+PRINT ARG=phi,psi FILE=COLVAR_DEMUX
diff --git a/user-doc/tutorials/trieste-5/.solutions/plumed2_reweight.dat b/user-doc/tutorials/trieste-5/.solutions/plumed2_reweight.dat
index d729a1a14..3b9ab7647 100644
--- a/user-doc/tutorials/trieste-5/.solutions/plumed2_reweight.dat
+++ b/user-doc/tutorials/trieste-5/.solutions/plumed2_reweight.dat
@@ -24,7 +24,7 @@ metad: METAD ...
 ...
 
 # Print both collective variables and the value of the bias potential on COLVAR_CONCAT file
-PRINT ARG=phi,psi FILE=COLVAR_CONCAT STRIDE=10
+PRINT ARG=phi,psi FILE=COLVAR_CONCAT
 
 # Then write the bias potential (as we did for reweighting)
 # As a good practice, remember that there might be multiple bias
diff --git a/user-doc/tutorials/trieste-5/.solutions/plumed3.dat b/user-doc/tutorials/trieste-5/.solutions/plumed3.dat
new file mode 100644
index 000000000..ef90a0b4a
--- /dev/null
+++ b/user-doc/tutorials/trieste-5/.solutions/plumed3.dat
@@ -0,0 +1,24 @@
+# load the pdb to better select torsions later
+MOLINFO STRUCTURE=reference.pdb
+# Compute the backbone dihedral angle phi, defined by atoms C-N-CA-C
+phi: TORSION ATOMS=@phi-2
+# Compute the backbone dihedral angle psi, defined by atoms N-CA-C-N
+psi: TORSION ATOMS=@psi-2
+
+metad: METAD ...
+# Activate well-tempered metadynamics
+# Deposit a Gaussian every 500 time steps, with initial height equal
+# to 1.2 kJoule/mol, biasfactor equal to 10.0
+# Remember: replica 0 and 1: no bias
+# replica 2, bias on psi
+  ARG=@replicas:{phi,psi,psi}
+  HEIGHT=@replicas:{0.0,0.0,1.2} # make sure that replicas 0 and 1 feel no potential!
+  PACE=500
+  BIASFACTOR=10.0
+  SIGMA=0.35
+# Gaussians will be written to file and also stored on grid
+  FILE=HILLS GRID_MIN=-pi GRID_MAX=pi
+...
+
+# Print both collective variables and the value of the bias potential on COLVAR file
+PRINT ARG=phi,psi FILE=COLVAR STRIDE=10
diff --git a/user-doc/tutorials/trieste-5/.solutions/plumed3_reweight.dat b/user-doc/tutorials/trieste-5/.solutions/plumed3_reweight.dat
new file mode 100644
index 000000000..cdd52a5f9
--- /dev/null
+++ b/user-doc/tutorials/trieste-5/.solutions/plumed3_reweight.dat
@@ -0,0 +1,35 @@
+# load the pdb to better select torsions later
+MOLINFO STRUCTURE=reference.pdb
+# Compute the backbone dihedral angle phi, defined by atoms C-N-CA-C
+phi: TORSION ATOMS=@phi-2
+# Compute the backbone dihedral angle psi, defined by atoms N-CA-C-N
+psi: TORSION ATOMS=@psi-2
+
+metad: METAD ...
+# Activate well-tempered metadynamics
+# Deposit a Gaussian every 500 time steps, with initial height equal
+# to 1.2 kJoule/mol, biasfactor equal to 10.0
+# Remember: replica 0 and 1: no bias
+# replica 2, bias on psi
+  ARG=@replicas:{phi,psi,psi}
+  HEIGHT=0
+#  PACE=500
+  BIASFACTOR=10.0
+  SIGMA=0.35
+  FILE=HILLS GRID_MIN=-pi GRID_MAX=pi
+  RESTART=YES
+  TEMP=300
+  PACE=100000000
+...
+
+# Print both collective variables and the value of the bias potential on COLVAR_CONCAT file
+PRINT ARG=phi,psi FILE=COLVAR_CONCAT
+
+# Then write the bias potential (as we did for reweighting)
+# As a good practice, remember that there might be multiple bias
+# potential applied (e.g. METAD and a RESTRAINT).
+# to be sure that you include all of them, just combine them:
+bias: COMBINE ARG=*.bias PERIODIC=NO
+# here omit STRIDE, so that you will print the bias
+# for all the frames in your concatenated trajectory
+PRINT FILE=COLVAR_WHAM ARG=bias
-- 
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